Department of Organismal Biology

Genome Engineering Zebrafish National Facility

Genome engineering zebrafish national facility provides a top-quality tailor-made service for our users, combining cutting-edge techniques with expert guidance and support.

Nordic Zebrafish and Husbandry meeting 2018

Nordic Zebrafish and Husbandry meeting 2018


Stockholm          November 7-9,  2018

For more information, please click here.

About the facility

Zebrafish embryos are transparent and develop outside the mother’s body, which greatly facilitates manipulation and imaging of biological processes. The facility allows researchers to take advantage of these unique features and provides initial advice on feasibility and experimental design as well as running support over the course of the project.

The facility, financed by both SciLifeLab and the University’s faculty of Science and Technology, provides a top-quality tailor-made service for local and national users, combining cutting-edge techniques with expert guidance and support to allow researchers to achieve optimal results.

Services and Methods

The genome engineering zebrafish facility offers a wide range of services at national and local levels.

National services:

  • Testing of CRIPSR gRNA activity and genotyping optimization
  • Single and multiplex CRISPR/Cas9 genome modification in wild type or reporter lines
  • Tol2 transgenesis and development of the site-specific DNA insertions
  • Chemical exposure of zebrafish larvae
  • Project planning/management

Local services:

  • Access to a well-equipped facility including tank systems, fish lines, workstations, incubators and injectors
  • Embryo and adult fish production
  • Zebrafish caretaking
  • Educational events
  • Monthly Uppsala Zebrafish Forum meetings
  • The latest Genome Engineering Zebrafish Newsletter is available here.

For detailed offer press here.

Equipment

  • Fish facility. Including tank systems, fish lines, workstations, incubators and injectors
  • Large scale robotic expression analysis Intavis InsituPro Vsi.
  • Microscopy. Variety of microscopy equipment (including fluorescent, confocal and electron microscopy). Lightsheet microscopy (Zeiss Lightsheet Z.1) in collaboration with BioVis

Follow the link for a more comprehensive list of equipment.