Full details of the offers are available in this PDF.
Zebrafish CRISPR Knock-Out (KO) Service
Promotor, regulatory elements and exon deletions can be ordered at an extra cost.
Zebrafish CRISPR Knock-In Service
Standard service includes insertion of the GFP protein under the expression of the endogenous gene promoter. Other methods can be applied at the customized pricing model.
Life Imaging with high throughput automated VAST system (Union Biometrica)
Embryo and larvae 3-6 days post fertilization, 96-well plate based, bright field and fluorescent (green and red) imaging, magnification: 4X, 5X, 10X, (20X), automated positioning of the fish, image analysis option
Flat rate of 1000 sek/run + 250 sek/h of the run
Fish embryo acute toxicity (FET)
Test based on the adapted Test Guideline 236. This test is designed to determine acute toxicity of chemicals on embryonic stages of fish. Recommended by the EU grant committee as an intermediate step between in vitro and in vivo mouse studies.
Evaluation of the human cancer cell line xenografts in the zebrafish embryo
An assay designed to evaluate cancer cell line compatibility with zebrafish model, including cell size, behavior, survival, engraftment side and embryo toxicity.
- Cell survival was tested in vivo at 33°C
- Cells are fluorescently labeled
- Cells are provided in 0.25-0.5x106 cells/ul concentration
Tumour growth & metastasis assay
Customized experimental set up based on the results of the evaluation assay, injections at 1 or 2 dpf into the blood flow, brain cavity or PVC space. Imaging of the xenotransplanted fish at 2 time points. Possibility of combining xenotransplantation procedure with the drug treatment.